Two-dimensional Protein Analysis by Isoelectric Focusing in a Multi-cell Plate System

Author(s): Beatriz Dos Santos Ferreira, Alan Trindade Branco, Karlla Salim de Queiroz, Goncalo Apolinario de Souza Filho.

Journal Name: Protein & Peptide Letters

Volume 9 , Issue 1 , 2002

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Abstract:

A practical and low cost system for isoelectric protein focusing (IEF) was developed. The system uses a multi-cell glass plate compatible with a common vertical one-dimensional electrophoresis chamber, dispensing specific IEF apparatus. After focusing, the IEF gels are easily recovered. The resulting two-dimensional (2-D) gels have provided efficient protein separation for different concentrations and extracts.

Keywords: isoelectric protein focusing (IEF), sodium dodecylsulfate (SDS)-PAGE, mercaptoethanol, Ampholytes solution, maize endosperm

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Article Details

VOLUME: 9
ISSUE: 1
Year: 2002
Page: [39 - 43]
Pages: 5
DOI: 10.2174/0929866023409057

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