A practical and low cost system for isoelectric protein focusing (IEF) was developed. The system uses a multi-cell glass plate compatible with a common vertical one-dimensional electrophoresis chamber, dispensing specific IEF apparatus. After focusing, the IEF gels are easily recovered. The resulting two-dimensional (2-D) gels have provided efficient protein separation for different concentrations and extracts.
Keywords: isoelectric protein focusing (IEF), sodium dodecylsulfate (SDS)-PAGE, mercaptoethanol, Ampholytes solution, maize endosperm
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