Co-Expression of GroEL/ES Enhances the Expression of Plant Catalase in Bacterial Cytosol

Author(s): Prosenjit Mondal, Mamata Ray, Sushmita Sahu, Surendra Chandra Sabat.

Journal Name: Protein & Peptide Letters

Volume 15 , Issue 10 , 2008

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Abstract:

Expression of plant proteins in E. coli is frequently unsuccessful, but soluble and functional rice catalase-B can be produced in E. coli when it is co-expressed with the chaperone GroEL/ES. The rice catalase exhibited properties typical for a catalase including the decomposition of H2O2 and inhibition by aminotriazole, a specific inhibitor for plant and animal catalases. This achievement records for first time the successful expression of a both native and variant rice plant catalase in bacterial cytosol suggesting that it may be an option to be considered for the expression of other plant proteins in E. coli.

Keywords: GroEL/ES, Soluble expression, western blotting, normal and variant rice catalase-B, Plant protein, Heterologous expression

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Article Details

VOLUME: 15
ISSUE: 10
Year: 2008
Page: [1075 - 1078]
Pages: 4
DOI: 10.2174/092986608786071058
Price: $58

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