Synthesis of Acyl Carrier Protein Using a New Heterocyclic Carboxyl Activating Group, 3-Mercapto-5,6-Diphenyl-1,2,4-Triazine Through SPPS Strategy

Author(s): M. P. Rajan, E. Purushothaman.

Journal Name: Protein & Peptide Letters

Volume 15 , Issue 10 , 2008

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Abstract:

A new, effective and easily accessible carboxyl activating group , 3-mercapto-5, 6 - diphenyl-1, 2, 4 - triazine was developed and the effectiveness was investigated using acyl derivatives, chemically by the preparation of respective amides, in addition to monitoring spectrophotometrically exploiting the UV- visible spectra. The carboxyl activating nature of the above mercapto heterocycle was further confirmed by solid phase peptide synthesis. Here CLPSER resin as polymeric support was applied. We synthesized Acyl Carrier Protein and smaller peptides by replacing this thiol for other conventional carboxyl activating reagents. These peptides synthesized were purified by HPLC and characterized by molecular weight method.

Keywords: Acyl Carrier Protein, Synthesis, SPPS Strategy, carboxyl activating group, UV- visible spectra, mercapto heterocycle, peptide synthesis, CLPSER resin, HPLC

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Article Details

VOLUME: 15
ISSUE: 10
Year: 2008
Page: [1068 - 1074]
Pages: 7
DOI: 10.2174/092986608786071148
Price: $58

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