Facile Conversion of FP to TR-FRET Assays using Terbium Chelates:Nuclear Receptor Competitive Binding Assays as Examples
Kurt W. Vogel,
Bryan D. Marks,
Kevin R. Kupcho,
Kevin L. Vedvik,
Tina M. Hallis.
Time-resolved FRET (TR-FRET) assays offer advantages over fluorescence polarization (FP) assays including reduced optical interference from compound autofluorescence or precipitation, and a greater ability to resolve potencies of tight-binding ligands. Here, we demonstrate the facile conversion of FP assays to a TR-FRET format using nuclear receptor ligand binding assays as examples.
Keywords: Fluorescence polarization, Time-resolved fret, Terbium chelates, Nuclear receptors
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