Purification and Characterization of Recombinant Lipid Storage Protein-2 from Drosophila melanogaster

Author(s): Estela L. Arrese, Laticia Rivera, Masakazu Hamada, Jose L. Soulages.

Journal Name: Protein & Peptide Letters

Volume 15 , Issue 9 , 2008

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Abstract:

Lipid storage protein 2 (Lsd 2) is a conserved insect protein that belongs to the small PAT family of proteins. PAT proteins are found associated to the lipid droplets of adipocytes and play significant roles in the regulation of triacylglycerides metabolism. Here we describe the expression and purification of Lsd2, its reconstitution in lipoprotein particles, the location of putative lipid binding sites and its secondary structure. This study provides the starting point for future studies on the mechanism of function of Lsd2. The similarities and differences between Lsd1 and Lsd2, the only PAT proteins found in insects, are discussed.

Keywords: Lipid storage droplet protein, adipocytes, fat body, Drosophila, lipolysis

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Article Details

VOLUME: 15
ISSUE: 9
Year: 2008
Page: [1027 - 1032]
Pages: 6
DOI: 10.2174/092986608785849191
Price: $65

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