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Protein & Peptide Letters
ISSN (Print): 0929-8665
ISSN (Online): 1875-5305
VOLUME: 15
ISSUE: 9
DOI: 10.2174/092986608785849281      Price:  $58









Strategies for Recombinant Expression of Small, Highly Disulphide- Bonded, Cationic Antimicrobial Peptides

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Author(s): A. L. Greenshields, L. C. Knickle, R. Syvitski and S. E. Douglas
Pages 985-994 (10)
Abstract:
Expression of two recombinant hepcidin homologues from Atlantic salmon, Salmo salar, characterization of their antimicrobial activity, and partial structural determination of the peptides is described. Expression was attempted in baculovirus and bacterial expression systems and the various purification and refolding methods used to determine the optimal strategy for production of active, correctly refolded hepcidin are reviewed.
Keywords:
Disulphide bond, refolding, recombinant expression, antimicrobial peptide, inclusion body
Affiliation:
Institute for Marine Biosciences, 1411 Oxford Street, Halifax, Nova Scotia, Canada B3H 3Z1.