Purification, Crystallization and Preliminary X-Ray Studies of AxCesD Required for Efficient Cellulose Biosynthesis in Acetobacter xylinum

Author(s): Min Yao, SongQing Hu, YongGui Gao, Kenji Tajima, Takanori Yoda, Daisuke Shimura, Yasuharu Satoh, Shin Kawano, Isao Tanaka, Masanobu Munekata.

Journal Name: Protein & Peptide Letters

Volume 15 , Issue 1 , 2008

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Abstract:

AxCesD protein required for bacterial cellulose biosynthesis in Acetobacter xylinum was overexpressed in E. coli, purified and crystallized. Single crystals of SeMet-substituted AxCesD were obtained by the sitting-drop vapordiffusion method. The crystal belongs to the primitive trigonal space group P32, with unit-cell parameters a = b = 77.7 Å, and c = 213.9 Å. The asymmetric unit in the crystal was assumed to contain 8 protein molecules giving the Matthews coefficient (VM ) of 2.54 Å3 Da-1. Se-MAD data were collected to 2.3 Å resolution using synchrotron radiations.

Keywords: AxCesD, bacterial cellulose, biosynthesis, crystal, Acetobacter xylinum

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Article Details

VOLUME: 15
ISSUE: 1
Year: 2008
Page: [115 - 117]
Pages: 3
DOI: 10.2174/092986608783330422

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