Title: Purification, Crystallization and Preliminary X-Ray Studies of AxCesD Required for Efficient Cellulose Biosynthesis in Acetobacter xylinum
VOLUME: 15 ISSUE: 1
Author(s):Min Yao, SongQing Hu, YongGui Gao, Kenji Tajima, Takanori Yoda, Daisuke Shimura, Yasuharu Satoh, Shin Kawano, Isao Tanaka and Masanobu Munekata
Affiliation:Faculty of Advanced Life Science, Hokkaido University, 060-0810, Sapporo, Japan.
Keywords:AxCesD, bacterial cellulose, biosynthesis, crystal, Acetobacter xylinum
Abstract: AxCesD protein required for bacterial cellulose biosynthesis in Acetobacter xylinum was overexpressed in E. coli, purified and crystallized. Single crystals of SeMet-substituted AxCesD were obtained by the sitting-drop vapordiffusion method. The crystal belongs to the primitive trigonal space group P32, with unit-cell parameters a = b = 77.7 Å, and c = 213.9 Å. The asymmetric unit in the crystal was assumed to contain 8 protein molecules giving the Matthews coefficient (VM ) of 2.54 Å3 Da-1. Se-MAD data were collected to 2.3 Å resolution using synchrotron radiations.
