Expression and Purification of CB2 for NMR Studies in Micellar Solution

Title: Expression and Purification of CB2 for NMR Studies in Micellar Solution

Volume: 14 Issue: 10

Author(s): Dmitriy Krepkiy, Klaus Gawrisch and Alexei Yeliseev

Affiliation:

Keywords: Cannabinoid receptor CB2, dodecylphosphocholine micelles, circular dichroism spectroscopy, 1H NMR, ligand binding

Abstract: We demonstrate feasibility of biophysical characterization of the peripheral cannabinoid receptor CB2 produced by heterologous expression in E. coli membranes. Recombinant receptor was purified by affinity chromatography, and NMR diffusion experiments performed on CB2 solubilized in dodecylphosphocholine (DPC) micelles. Circular dichroism spectroscopy indicated high α-helical content (49 %) of CB2.

Cite this article as:

Krepkiy Dmitriy, Gawrisch Klaus and Yeliseev Alexei, Expression and Purification of CB2 for NMR Studies in Micellar Solution, Protein & Peptide Letters 2007; 14 (10) . https://dx.doi.org/10.2174/092986607782541051