Abstract
Mycobacterium Tuberculosis HSP16.3 is a major antigen maximally expressed during the stationary phase. Previous studies showed that HSP16.3 can function as a molecular chaperone in vitro. Here, crystallization trails of HSP 16.3 were reported. A kind of crystal can be diffracted to 2.8 A resolution at the ”Photon Factory“, a synchrotron light source in Japan. The crystal displayed the space group R3 with unit cell parameters a=b=110 A , c=152 A and gamma=120 degree. Assuming the presence of 9 HSP16.3 molecules in an asymmetric unit, it gives a Vm= 0.73 A 3 / Da. and solvent content of 35percent by volume.
Keywords: MYCOBACTERIUM TUBERCULOSIS HSP16.3, IPTG isopropyl ß-D-thiogalacto-pyranoside, MPD 2-Methyl-2,4-Pentanediol, sHSP small heat shock protein, MAD Multiwavelength Anomalous Dispersion, molecular chaperone activity
Protein & Peptide Letters
Title: Preliminary X-Ray Crystallographic Studies Of The Mycobacterium Tuberculosis Hsp16.3 Molecular Chaperone
Volume: 8 Issue: 6
Author(s): Y. Chen, J. An, Y. Ding, H. Dai, Q. Mao, L. Feng, B. Liu, Y. Chang, F. Chen, H. He, H. Tang, Z. Chang and Z. Rao
Affiliation:
Keywords: MYCOBACTERIUM TUBERCULOSIS HSP16.3, IPTG isopropyl ß-D-thiogalacto-pyranoside, MPD 2-Methyl-2,4-Pentanediol, sHSP small heat shock protein, MAD Multiwavelength Anomalous Dispersion, molecular chaperone activity
Abstract: Mycobacterium Tuberculosis HSP16.3 is a major antigen maximally expressed during the stationary phase. Previous studies showed that HSP16.3 can function as a molecular chaperone in vitro. Here, crystallization trails of HSP 16.3 were reported. A kind of crystal can be diffracted to 2.8 A resolution at the ”Photon Factory“, a synchrotron light source in Japan. The crystal displayed the space group R3 with unit cell parameters a=b=110 A , c=152 A and gamma=120 degree. Assuming the presence of 9 HSP16.3 molecules in an asymmetric unit, it gives a Vm= 0.73 A 3 / Da. and solvent content of 35percent by volume.
Export Options
About this article
Cite this article as:
Chen Y., An J., Ding Y., Dai H., Mao Q., Feng L., Liu B., Chang Y., Chen F., He H., Tang H., Chang Z. and Rao Z., Preliminary X-Ray Crystallographic Studies Of The Mycobacterium Tuberculosis Hsp16.3 Molecular Chaperone, Protein & Peptide Letters 2001; 8 (6) . https://dx.doi.org/10.2174/0929866013409111
DOI https://dx.doi.org/10.2174/0929866013409111 |
Print ISSN 0929-8665 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5305 |
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
New Aminoacyl-tRNA Synthetase Inhibitors as Antibacterial Agents
Current Drug Targets - Infectious Disorders Silicosis in Current Scenario: A Review of Literature
Current Respiratory Medicine Reviews Protein Kinase Homology Models: Recent Developments and Results
Current Medicinal Chemistry Arylurea Derivatives: A Class of Potential Cancer Targeting Agents
Current Topics in Medicinal Chemistry Nicotinamide Supplementation Protects Gestational Diabetic Rats by Reducing Oxidative Stress and Enhancing Immune Responses
Current Medicinal Chemistry Dry Powder form of Polymeric Nanoparticles for Pulmonary Drug Delivery
Current Pharmaceutical Design subject Index To Volume 3
Current Drug Targets The Coronin Family and Human Disease
Current Protein & Peptide Science Internet Resources for Drug Discovery and Design
Current Topics in Medicinal Chemistry Bibliometric Assessment of the Latin-American Contributions in Dengue
Recent Patents on Anti-Infective Drug Discovery Liposomes: Targeted and Controlled Delivery System
Drug Delivery Letters Virtual Screening Strategies in Medicinal Chemistry: The State of the Art and Current Challenges
Current Topics in Medicinal Chemistry Treatment Failure in Ventilator Associated Pneumonia
Current Respiratory Medicine Reviews How Molecular Epidemiology Can Affect Tuberculosis Control in the Middle East Countries: A Systematic Review and Meta-Analysis
Infectious Disorders - Drug Targets Oral Factor Xa (FXa) Inhibitors for Treatment of Heparin-induced Thrombocytopenia (HIT)
Current Drug Therapy New Insights in Design and Development of Antitubercular Drugs
Current Bioactive Compounds Chemokines and Persistent Inflammation in Rheumatoid Arthritis: Hunting for Therapeutic Targets
Current Medicinal Chemistry - Anti-Inflammatory & Anti-Allergy Agents Antimicrobial Peptides: Mediators of Innate Immunity as Templates for the Development of Novel Anti-Infective and Immune Therapeutics
Current Pharmaceutical Design Tumor Necrosis Factor-a Blockade in Rheumatoid Arthritis: Responders Vs Non Responders
Medicinal Chemistry Reviews - Online (Discontinued) Vanadium Complexes with Hydrazone or Thiosemicarbazone Ligands as Potential Anti-Mycobacterium tuberculosis Agents
Current Clinical Pharmacology