A thermostable DNA photolyase from an extremely thermophilic bacterium, Thermus thermophilus HB8 has been crystallized by the hanging drop vapor diffusion method using NH4H2PO4 as a precipitant. The hexagonal crystals were grown to a size of 0.4 mm in length. X-ray diffraction experiments show the crystals to belong to the hexagonal space group P6 5 22 or P6 1 22 with the unit cell dimensions of a=b=113.6 A, c=142.0 A. They diffract X-rays to 2.4 A resolution with synchrotron radiation.
Keywords: THERMOSTABLE DNA PHOTOLYASE, THERMUS THERMOPHILUS HB8, CPD photolyase, Escherichia coli, Anacystis nidulans
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