Crystallization And Preliminary X-Ray Studies Of A Thermostable Dna Photolyase From Thermus Thermophilus Hb8

Author(s): Hirofumi Komori, Hiroshi Tsujiuchi, Ryoji Masuii, seiki Kuramitsu, Shigeyuki Yokoyama, Takehiko Shibata, Yorinao Inoue, Kunio Miki.

Journal Name: Protein & Peptide Letters

Volume 8 , Issue 6 , 2001

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Abstract:

A thermostable DNA photolyase from an extremely thermophilic bacterium, Thermus thermophilus HB8 has been crystallized by the hanging drop vapor diffusion method using NH4H2PO4 as a precipitant. The hexagonal crystals were grown to a size of 0.4 mm in length. X-ray diffraction experiments show the crystals to belong to the hexagonal space group P6 5 22 or P6 1 22 with the unit cell dimensions of a=b=113.6 A, c=142.0 A. They diffract X-rays to 2.4 A resolution with synchrotron radiation.

Keywords: THERMOSTABLE DNA PHOTOLYASE, THERMUS THERMOPHILUS HB8, CPD photolyase, Escherichia coli, Anacystis nidulans

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Article Details

VOLUME: 8
ISSUE: 6
Year: 2001
Page: [495 - 498]
Pages: 4
DOI: 10.2174/0929866013409157
Price: $58

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