Functional Expression of a Galactose-Binding C-Type Lectin In Pichia pastoris

Author(s): Jia-da Li, Qiang Xu, Ke-yi Wang.

Journal Name: Protein & Peptide Letters

Volume 8 , Issue 3 , 2001

Abstract:

Trimeresurus stejnegeri lectin (TSL) was expressed in the yeast Pichia pastoris under the control of alcohol oxidase (AOX1) promoter. The recombinant protein, which was glycosylated, can be purified by one-step affinity chromatography. The purified recombinant TSL shared similar sugar-binding activity and Specificity with the native protein as determined by hemagglutination and enzyme-linked lectin binding Assays.

Keywords: C-TYPE LECTIN, PICHIA PASTORIS, Trimeresurus stejnegeri lectin (TSL), alcohol oxidase (AOX1) promoter, galactose-binding C-type, mutagenesis, alcohol oxidase (AOX1)

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Article Details

VOLUME: 8
ISSUE: 3
Year: 2001
Page: [217 - 224]
Pages: 8
DOI: 10.2174/0929866013409463
Price: $58

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