Functional Expression of a Galactose-Binding C-Type Lectin In Pichia pastoris

Author(s): Jia-da Li, Qiang Xu, Ke-yi Wang.

Journal Name: Protein & Peptide Letters

Volume 8 , Issue 3 , 2001


Trimeresurus stejnegeri lectin (TSL) was expressed in the yeast Pichia pastoris under the control of alcohol oxidase (AOX1) promoter. The recombinant protein, which was glycosylated, can be purified by one-step affinity chromatography. The purified recombinant TSL shared similar sugar-binding activity and Specificity with the native protein as determined by hemagglutination and enzyme-linked lectin binding Assays.

Keywords: C-TYPE LECTIN, PICHIA PASTORIS, Trimeresurus stejnegeri lectin (TSL), alcohol oxidase (AOX1) promoter, galactose-binding C-type, mutagenesis, alcohol oxidase (AOX1)

Rights & PermissionsPrintExport Cite as

Article Details

Year: 2001
Page: [217 - 224]
Pages: 8
DOI: 10.2174/0929866013409463
Price: $58

Article Metrics

PDF: 2