Crystallization and Preliminary X-Ray Crystallographic Studies of Human Autocrine Motility Factor

Author(s): Hiroshi Uemura, Nobutada Tanaka, Tatsuyshi Funasaka, Arayo Haga, Hisamitsu Nagase, Avraham Raz, Kazuo T. Nakamura.

Journal Name: Protein & Peptide Letters

Volume 8 , Issue 4 , 2001

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Abstract:

Human autocrine motility factor (hAMF), a tumor-secreted cytokine which stimulates cell migration in vitro and metastasis in vivo, has been crystallized by the hanging-drop vapour diffusion method. The crystals belong to an orthorhombic space group P212121 with the cell dimensions of a = 80.79 A, b = 107.1 A, and c = 270.9 A. There are two dimers per asymmetric unit. The crystals diffract to at least 2.0 A resolution and are suitable for X-ray structure analysis at high resolution.

Keywords: Crystallization, Motility Factor, cytokine

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Article Details

VOLUME: 8
ISSUE: 4
Year: 2001
Page: [317 - 322]
Pages: 6
DOI: 10.2174/0929866013409364
Price: $58

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