Abstract
Secondary structure composition of two homologous proteins, alpha-fetoprotein and serum albumin, has been compared using circular dichroism and Fourier-transform infrared spectroscopy. CD-only analysis gives an accurate a-helical content for SA, but underestimates the predicted value for AFP. In contrast, FTIR-only analysis underestimates the helical content of SA. However, analysis of hybrid IR-CD spectra gives more accurate values for secondary structure composition for both AFP and SA and shows that the secondary structures of these proteins are the same.
Keywords: Proteins Fetoprotein, Serum Albumin, Circular Dichroism, Human serum albumin (HSA)
Protein & Peptide Letters
Title: Secondary Structure of the Homologous Proteins, A A-Fetoprotein and Serum Albumin, from their Circular Dichroism and Infrared Spectra
Volume: 8 Issue: 4
Author(s): Keith A. Oberg and Vladimir N. Uversky
Affiliation:
Keywords: Proteins Fetoprotein, Serum Albumin, Circular Dichroism, Human serum albumin (HSA)
Abstract: Secondary structure composition of two homologous proteins, alpha-fetoprotein and serum albumin, has been compared using circular dichroism and Fourier-transform infrared spectroscopy. CD-only analysis gives an accurate a-helical content for SA, but underestimates the predicted value for AFP. In contrast, FTIR-only analysis underestimates the helical content of SA. However, analysis of hybrid IR-CD spectra gives more accurate values for secondary structure composition for both AFP and SA and shows that the secondary structures of these proteins are the same.
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Cite this article as:
Oberg A. Keith and Uversky N. Vladimir, Secondary Structure of the Homologous Proteins, A A-Fetoprotein and Serum Albumin, from their Circular Dichroism and Infrared Spectra, Protein & Peptide Letters 2001; 8 (4) . https://dx.doi.org/10.2174/0929866013409391
DOI https://dx.doi.org/10.2174/0929866013409391 |
Print ISSN 0929-8665 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5305 |
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