The Native Gene of Anti-Lps Factor from Tachypleus tridentatus: Cloning, Expression and its Bacteriostatic Activity in Vitro | BenthamScience

The Native Gene of Anti-Lps Factor from Tachypleus tridentatus: Cloning, Expression and its Bacteriostatic Activity in Vitro

Author(s): Dong-Ning Wang, Lin Chen, Jie-Wu Liu, Zhi-Yong He, Wei-Jie Zhang, Xiang-Fu Wu.

Journal Name: Protein & Peptide Letters

Volume 8 , Issue 4 , 2001

Abstract:

The native gene information of anti-LPS factor from Tachypleus tridentatus was first reported in this study. The total RNA was extracted from amebocytes of Tachypleus tridentatus and the first-strand cDNA was synthesized by RT-PCR. Then the amplified cDNA of TALF was cloned and expressed in Pichia pastoris. The biological activity of the recombinant proteins obtained have been confirmed by LPS neutralization assay and bacteriostatic assay in vitro.

Keywords: ANTI-LPS FACTOR, TACHYPLEUS TRIDENTATUS, CLONING, EXPRESSION, BACTERIOSTATIC ACTIVITY, Lipopolysaccharide (LPS), Endotoxin, Limulus polyphemus, Degenerate Oligonucleotides, LPS neutralization assay, Bacteriostatic assay

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Article Details

VOLUME: 8
ISSUE: 4
Year: 2001
Page: [273 - 280]
Pages: 8
DOI: 10.2174/0929866013409337
Price: $58

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