DETECTION AND PURIFICATION OF INSECTICIDAL PEPTIDES FROM SCORPIONS AND SPIDERS: A RAPID METHOD FOR THEIR ISOLATION FROM THEIR CRUDE VENOMS USING MALDI-TOF-MS ANALYSIS

Author(s): Gerardo Corzo, Elba Villegas, Hon-Seok Lee, Terumi Nakajima.

Journal Name: Protein & Peptide Letters

Volume 8 , Issue 5 , 2001

Abstract:

The insecticidal neurotoxins AaIT, LqhIT2, and miew-agaIV from venom of Arachnida were rapidly re-purified from their respective crude venoms using mass spectrometry analyses and two high pressure chromatographic steps. The purity of each toxin was confirmed by capillary zone electrophoresis. Each toxin was identified by mass spectrometry and their biological activity confirmed by bioassays against tobacco cutworms and mice. In addition, AaIT and LqhIT2 were partially sequenced using post-source decay mass spectrometry analysis. The spider neurotoxin miew-agaIV was identified from other isomassic peptide by fingerprint mass analysis of its tryptic fragments.

Keywords: INSECTICIDAL PEPTIDES, MALDI-TOF-MS, insecticidal neurotoxins AaIT, LqhIT, mass spectrometry, capillary zone electrophoresis, Androctonus australis, Leiurus quinquestriatus, Agelenopsis aperta

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Article Details

VOLUME: 8
ISSUE: 5
Year: 2001
Page: [375 - 383]
Pages: 9
DOI: 10.2174/0929866013409238
Price: $58

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