Crystallization And Preliminary X-Ray Diffraction Study Of Riboflavin Synthase

Author(s): Zdzislaw Wawrazak, Paul V. Viitanen, Joseph C. Calabrese, Douglas B. Jordan.

Journal Name: Protein & Peptide Letters

Volume 8 , Issue 1 , 2001

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Abstract:

Escherichia coli riboflavin synthase crystallizes at 22 C in the presence of 7-10percent by volume diglyme, 20-50 mM MgCl2 and pH 7.0. In this medium diffraction quality crystals are routinely obtained within 5 h and they are stable for 10 weeks. The crystals are orthogonal in space group P212121 with unit cell dimensions of a=52.4 A , b = 62.1 A, c = 218.8 A. A 97percent complete data set was collected at 2.1 A resolution.

Keywords: RIBOFLAVIN SYNTHASE

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Article Details

VOLUME: 8
ISSUE: 1
Year: 2001
Page: [69 - 73]
Pages: 5
DOI: 10.2174/0929866013409698

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