Crystallization And Preliminary X-Ray Studies Of L-(+)-2,3-Butanediol Dehydrogenase From Brevibacterium Saccharolyticum C-1012

Author(s): Masato Otagiri, Genji Kurisu, Sadaharu Ui, Moriya Ohkum, Toshiaki Kudo, Masami Kusunoki.

Journal Name: Protein & Peptide Letters

Volume 8 , Issue 1 , 2001

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Abstract:

L(+)-2,3 -Butanediol dehydrogenase (L-BDH) from Brevibacterium saccharolyticum C-1012 has been crystallized by the hanging drop vapor diffusion method with polyethylene glycol 4000 as the precipitant. Crystals of L-BDH belong to the triclinic system, space group P1 with cell dimensions a = 60.8 A, b = 69.2 A, c = 127.4 A , alpha = 96.1 , beta = 100.2 and gama=109.6 . Crystals diffracted to 2.0 A resolution on a synchrotron radiation, and a full data set was collected at resolution of 2.0 A at 100K.c

Keywords: BUTANEDIOL DEHYDROGENASE, BREVIBACTERIUM SACCHAROLYTICUM, Klebsiella pneumoniae, Bacillus polymyxa, Serratia marcescens, Three-dimensional structures

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Article Details

VOLUME: 8
ISSUE: 1
Year: 2001
Page: [57 - 61]
Pages: 5
DOI: 10.2174/0929866013409670

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