Expression Of The N-Terminal Segment Of Qbrn-2 In E. Coli And Tips On Preparation Of A Recombinant Protein

Title: Expression Of The N-Terminal Segment Of Qbrn-2 In E. Coli And Tips On Preparation Of A Recombinant Protein

Volume: 8 Issue: 1

Author(s): Wei Liu, Rongqaio He and Zhigang Xue

Affiliation:

Keywords: E.COLI, quail neurogenesis, myogenesis

Abstract: POU box gene qBrn -2 exhibits restricted expression pattern in quail neurogenesis and myogenesis. Here we report isolation of a N-terminal segment (P207) of qBrn-2 expressed in E. coli and tips on preparation of a recombinant protein. Recombinant P207 was purified and migrated as a single band on SDS-PAGE with the same apparent molecular mass as predicted. In the expression of P207 by pET 3b vector, IPTG concentration for induction greatly affected the expression level. Further, fusion domain at N-terminus seemed to change the stability of P207. Those results suggest some useful tips on preparation of a recombinant protein.

Cite this article as:

Liu Wei, He Rongqaio and Xue Zhigang, Expression Of The N-Terminal Segment Of Qbrn-2 In E. Coli And Tips On Preparation Of A Recombinant Protein, Protein & Peptide Letters 2001; 8 (1) . https://dx.doi.org/10.2174/0929866013409733