POU box gene qBrn -2 exhibits restricted expression pattern in quail neurogenesis and myogenesis. Here we report isolation of a N-terminal segment (P207) of qBrn-2 expressed in E. coli and tips on preparation of a recombinant protein. Recombinant P207 was purified and migrated as a single band on SDS-PAGE with the same apparent molecular mass as predicted. In the expression of P207 by pET 3b vector, IPTG concentration for induction greatly affected the expression level. Further, fusion domain at N-terminus seemed to change the stability of P207. Those results suggest some useful tips on preparation of a recombinant protein.
Keywords: E.COLI, quail neurogenesis, myogenesis
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