A Direct Continuous pH-Spectrophotometric Assay for Arginine Kinase Activity

Author(s): Yu Z., Pan J..

Journal Name: Protein & Peptide Letters

Volume 9 , Issue 6 , 2002

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Abstract:

A direct and continuous spectrophotometric method was developed for determining arginine kinase (phosphoarginine synthesis) activity. Protons are produced during the phosphoarginine synthesis course, so adding the complex acid-base indicator to this solution and monitoring the decrease of absorbance of the solution at 575 nm will follow the arginine kinase activity. For this condition, one activity unit of arginine kinase was defined as 1 μmol H+ produced in 1 minute in the enzymatic reaction catalyzed by arginine kinase.

Keywords: arginine kinase, continuous assay, enzyme activity, ph-spectrophotometric

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Article Details

VOLUME: 9
ISSUE: 6
Year: 2002
Page: [545 - 552]
Pages: 8
DOI: 10.2174/0929866023408382
Price: $58

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