Biochemical Characterization of Apoptotic Cleavage of KH-Type Splicing Regulatory Protein (KSRP) / Far Upstream Element-Binding Protein 2 (FBP2)

Author(s): Heeyoung Seok, Jinsun Cho, Minseok Cheon, II-Seon Park.

Journal Name: Protein & Peptide Letters

Volume 9 , Issue 6 , 2002

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Abstract:

Caspases, Asp-specific cysteine protease, cleave proteins upon apoptosis. To identify and characterize new caspase substrate in the nucleus, the proteome of the rat liver extracts was analyzed after the treatment with caspases. One of the identified proteins was KSRP / FBP2 that is preferentially cleaved by caspase-3 and -7 at two sites after Asp102 and Asp183. The second site was cleaved only in the protein produced in cells, but not in in vitro translated protein. These results indicate that more than the primary sequence may be important for the recognition by caspases.

Keywords: ksrp, caspases, proteome, substrate specificity

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Article Details

VOLUME: 9
ISSUE: 6
Year: 2002
Page: [511 - 519]
Pages: 9
DOI: 10.2174/0929866023408454
Price: $58

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