Selective Precipitation of Haptoglobin and α2-Macroglobulin from Human Serum Using Alocasia macrorhiza Tuber Protein

Author(s): B. Shivananda Nayak, N. Jagadish Ulloor, B. Shivaraj.

Journal Name: Protein & Peptide Letters

Volume 9 , Issue 6 , 2002

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Abstract:

Treatment of human serum with ammonium sulfate fraction (0-50%) of Alocasia macrorhiza tuber extract resulted in precipitation at neutral pH. The precipitate was dissolved at pH 10.5 and chromatographed on Sephadex G-100 column. Two protein peaks were resolved. While the first peak represented α2-macroglobulin and haptoglobin, the second peak accounted for specific Alocasia protein. Incidentally the Alocasia protein was shown to be responsible for selective and specific precipitation of α2-macroglobulin and haptoglobin from serum. Thus the plant protein in its pure form or in crude stage could be used for the rapid isolation of two of the prominent a2-globulins.

Keywords: alocasia macrorhiza, macroglobulin, haptoglobin, alocasia protein, alocasia specific serum protein

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Article Details

VOLUME: 9
ISSUE: 6
Year: 2002
Page: [503 - 510]
Pages: 8
DOI: 10.2174/0929866023408391
Price: $58

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