Crystallization and Preliminary X-Ray Studies of the Fab Fragment from a Humanized Version of the Mouse Anti-Human Fas Antibody Hfe7a

Author(s): Shuichiro Ito, Tomoko Takayama, Hiroyuki Hanzawa, Tohru Takahashi, Kenji Miyadai, Nobufusa Serizawa, Hideyuki Haruyama, Tadashi Hata.

Journal Name: Protein & Peptide Letters

Volume 9 , Issue 3 , 2002

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Abstract:

A humanized version of the apoptosis-inducing mouse anti-human Fas monoclonal antibody, HFE7A, is under further development for the treatment of autoimmune diseases such as rheumatoid arthritis. We have crystallized the antigen-binding fragment (Fab) of the humanized HFE7A. The crystals belong to the orthorhombic space group P2 1 2 1 2 1 with cell dimensions a = 54.4 Å, b = 82.7 Å, c = 104.9 Å and contain one Fab molecule in the asymmetric unit. X-ray diffraction data were collected to 2.8 Å resolution.

Keywords: fab fragment, humanized hfe7a, fas monoclonal antibody

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Article Details

VOLUME: 9
ISSUE: 3
Year: 2002
Page: [259 - 263]
Pages: 5
DOI: 10.2174/0929866023408715
Price: $58

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