Conformational Analysis of Sea Cucumber (Caudina Arenicola) C Globin 94-97 Fragment, Pro-Glu-Leu-Leu

Author(s): P.N. Sunil Kumar, K. S. Devaky, C. Sadasivan, M. Haridas.

Journal Name: Protein & Peptide Letters

Volume 9 , Issue 5 , 2002

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The tetrapeptide Pro-Glu-Leu-Leu forms the 94-97 fragment of C globin in sea cucumber. 2% Butanediol dimethacrylate-cross linked polystyrene (2% BDDMA-PS), which had been optimized, was used for the synthesis of the tetrapeptide Pro-Glu-Leu-Leu. The peptide was synthesized by using Boc-amino acid strategy. The peptide purity was checked by RP-HPLC and the peptide was characterized by ¹H NMR spectroscopy and amino acid analysis. Conformation of the peptide was studied by 1D- and 2D- homonuclear ¹H NMR, in DMSO-d6 at 300K. The conformation of the synthetic tetrapeptide (extended backbone conformation) is not in agreement with that in C globin.

Keywords: Conformational analysis, sea cucumber, C globin, two-dimensional NMR

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Article Details

Year: 2002
Page: [403 - 409]
Pages: 7
DOI: 10.2174/0929866023408526
Price: $58

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