Kinetic Analysis of the Cleavage of Human Protease-Activated Receptor-1 / 2 / 3 and 4 Using Quenched-Fluorescent Peptide Substrates

Author(s): Mark T. Fox, Brett Greer, Jill Lawson, Adrienne Healy, Patrick Harriott.

Journal Name: Protein & Peptide Letters

Volume 9 , Issue 5 , 2002

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Protease-activated receptors [PARs] are a family of G-protein-coupled seven-transmembrane domain receptors that are activated by proteolytic cleavage of their amino-terminal exodomain. To characterize the cleavage rate of human PAR-1 / 2 / 3 and 4 by trypsin and thrombin, four synthetic quenched-fluorescent peptide substrates have been synthesized. Each substrate consisted of a ten-residue peptide spanning the receptor activation cleavage site and using progress-curve kinetics, kcat / Km values were determined.

Keywords: Enzymology, serine proteases, progress-curve kinetics, fluorescence, 2-aminobenzoyl (Abz), 2,4-dinitrophenyl (Dnp), fluorescence resonance, G-protein-coupled, seven-transmembrane, domain receptor

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Article Details

Year: 2002
Page: [387 - 393]
Pages: 7
DOI: 10.2174/0929866023408490
Price: $58

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