The use of Dodt as a Non-Malodorous Scavenger in Fmoc-Based Peptide Synthesis

A.      Teixeira; W.   E.   Benckhuijsen; P.   E.   de Koning; A.   R.P.M.   Valentijn; J.   W.   Drijfhout

Abstract

We have synthesized a random group of peptides and performed cleavages using various cleavage cocktails including 3,6-dioxa-1,8-octanedithiol (DODT). Purity of the peptides was compared to that obtained with standard protocols for cleavage using RP-HPLC and Maldi-Tof mass spectrometry. We show that stinking thiols can be replaced by the almost odourless (DODT) without negatively affecting the purity of the end product.

Keywords: NON-MALODOROUS, SCAVENGER, FMOC-BASED PEPTIDE, Peptide synthesis, thiols, DODT, 3,6-dioxa -1,8-octa ne dithiol

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Protein & Peptide Letters

Title: The use of Dodt as a Non-Malodorous Scavenger in Fmoc-Based Peptide Synthesis

Volume: 9 Issue: 5

Author(s): A. Teixeira, W. E. Benckhuijsen, P. E. de Koning, A. R.P.M. Valentijn and J. W. Drijfhout

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Keywords: NON-MALODOROUS, SCAVENGER, FMOC-BASED PEPTIDE, Peptide synthesis, thiols, DODT, 3,6-dioxa -1,8-octa ne dithiol

Abstract: We have synthesized a random group of peptides and performed cleavages using various cleavage cocktails including 3,6-dioxa-1,8-octanedithiol (DODT). Purity of the peptides was compared to that obtained with standard protocols for cleavage using RP-HPLC and Maldi-Tof mass spectrometry. We show that stinking thiols can be replaced by the almost odourless (DODT) without negatively affecting the purity of the end product.

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Teixeira A., Benckhuijsen E. W., de Koning E. P., Valentijn R.P.M. A. and Drijfhout W. J., The use of Dodt as a Non-Malodorous Scavenger in Fmoc-Based Peptide Synthesis, Protein & Peptide Letters 2002; 9 (5) . https://dx.doi.org/10.2174/0929866023408481