Protein & Peptide Letters

Prof. Ben M. Dunn  
Department of Biochemistry and Molecular Biology
University of Florida
College of Medicine
P.O. Box 100245
Gainesville, FL


Low Content of Protein S29 in Ribosomes of Human Lung Cancer Cell Line A549: Detected by Twodimensional Electrophoresis

Author(s): Zhi-Dong Zhou, Lei Bao, Ding-Gan Liu, Min-Qian Li, Yin-Zi Ge, Ying-Ling Huang and Wang-Yi Liu

Affiliation: State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, ShanghaiInstitutes of Biological Sciences, Chinese Academy of Sciences, 320 Yue-Yang Road, Shanghai 200031,China, Laboratory of Nanobiology and Nanomedicine, Shanghai Institute of Nuclear Research, Chinese Academy of Sciences, 2019 Jia-Luo Road, Shanghai 201800, China.


This study revealed that the content of protein S29 in ribosomes of cancer cell line A549 was distinctly low (equivalent to about 30% of that of 2BS). The conclusion was acquired based on the ratios of spot volume of ribosomal protein S29 to that of several other ribosomal proteins (S29 / L37a, S29 / L38, S29 / S27 and S29 / S28) in the same gel plate. The possible biological roles of ribosomal protein S29 in malignant transformation and translation regulation are briefly discussed.

Keywords: human liver cancer cell line 7721, human lung cancer cell line a549, human lung normal cell line 2bs, human lung cancer, northern blot, ribosomal protein s29, 2-dimensional polyacrylamide gel electrophoresis, cbb-chromium modified thiosulfate-silver staining

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Article Details

Page: [91 - 97]
Pages: 7
DOI: 10.2174/0929866033408273