Low Content of Protein S29 in Ribosomes of Human Lung Cancer Cell Line A549: Detected by Twodimensional Electrophoresis
Zhi-Dong Zhou, Lei Bao, Ding-Gan Liu, Min-Qian Li, Yin-Zi Ge, Ying-Ling Huang and Wang-Yi Liu
Affiliation: State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, ShanghaiInstitutes of Biological Sciences, Chinese Academy of Sciences, 320 Yue-Yang Road, Shanghai 200031,China, Laboratory of Nanobiology and Nanomedicine, Shanghai Institute of Nuclear Research, Chinese Academy of Sciences, 2019 Jia-Luo Road, Shanghai 201800, China.
This study revealed that the content of protein S29 in ribosomes of cancer cell line A549 was distinctly low (equivalent to about 30% of that of 2BS). The conclusion was acquired based on the ratios of spot volume of ribosomal protein S29 to that of several other ribosomal proteins (S29 / L37a, S29 / L38, S29 / S27 and S29 / S28) in the same gel plate. The possible biological roles of ribosomal protein S29 in malignant transformation and translation regulation are briefly discussed.
Keywords: human liver cancer cell line 7721, human lung cancer cell line a549, human lung normal cell line 2bs, human lung cancer, northern blot, ribosomal protein s29, 2-dimensional polyacrylamide gel electrophoresis, cbb-chromium modified thiosulfate-silver staining
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