Kinetic and Site-Directed Mutagenesis Studies of Prevotella Intermedia Acid Phosphatase

Author(s): Xiaochi Chen, Toshihiro Ansai, Sailen Barik, Tadamichi Takehara.

Journal Name: Protein & Peptide Letters

Volume 10 , Issue 1 , 2003

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Abstract:

Site-directed mutagenesis was used to examine the roles of the conserved histidine, arginine and cysteine residues in acid phosphatase from Prevotella intermedia (PiACP). The replacement of histidine and arginine residues resulted in the elimination of the PiACP activity while the cysteine mutants retained activity. These results suggest that the histidine and arginine residues are essential for catalysis.

Keywords: prevotella intermedia acp, piacp, acid phosphatase, site-directed mutagenesis

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Article Details

VOLUME: 10
ISSUE: 1
Year: 2003
Page: [53 - 59]
Pages: 7
DOI: 10.2174/0929866033408282

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