Site-Directed Mutagenesis and Preliminary X-Ray Crystallographic Studies of the Tabtoxin Resistance Protein

Author(s): Yi Ding, Shentao Li, Xiaofeng Li, Fei Sun, Jinyuan Liu, Nanming Zhao, Zihe Rao.

Journal Name: Protein & Peptide Letters

Volume 10 , Issue 3 , 2003

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Tabtoxin resistance protein (TTR) is an enzyme that catalyzes the acetylation of tabtoxin rendering tabtoxin-producing pathogens tolerant to their own phytotoxins. According to the structure based detoxification mechanism of TTR, three site-directed mutants Y141F, D130N and Y141F-D130N were constructed and overexpressed in E. coli. The products were then purified and their properties were analyzed by CD and DLS. Crystallization trials of two mutants Y141F andY141F-D130N were preformed.

Keywords: tabtoxin resistance protein, mutation, crystallization, circular dichroism spectra, dynamic light scattering

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Article Details

Year: 2003
Page: [255 - 263]
Pages: 9
DOI: 10.2174/0929866033478924
Price: $58

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