Crystallization And Preliminary X-Ray Analysis Of Class Ii Fructose-1,6-Bisphosphate Aldolase From Thermus Caldophilus

Author(s): Jun Hyuck Lee, Young Jun Im, Seong-Hwan Rho, Seong Ho Park, Mun-Kyoung Kim, Su Jin Cho, Tae-Yeon Kim, Jin Hwan Oh, Hyun-Jae Shin, Dae-Sil Lee, Soo Hyun Eom.

Journal Name: Protein & Peptide Letters

Volume 10 , Issue 5 , 2003

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Abstract:

In this study, we have crystallized class II fructose-1,6-bisphosphate aldolase (FBA) from Thermus caldophilus (Tca). Purified Tca FBA is a tetrameric enzyme of 305 residues, which crystallizes in the space group P212121 (cell dimensions a = 98.9, b = 113.1, c = 115.7 Å), with four molecules in the asymmetric unit. A complete diffraction data set was obtained from orthorhombic crystals at resolution of 2.2 Å.

Keywords: fructose, bisphosphate aldolase, dihydroxyaceton phosphate, glyceraldehydes-3-phosphate, thermus caldophilus

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Article Details

VOLUME: 10
ISSUE: 5
Year: 2003
Page: [511 - 515]
Pages: 5
DOI: 10.2174/0929866033478735
Price: $58

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