New Chemical Crosslinking Methods for the Identification of Transient Protein-Protein Interactions with Multiprotein Complexes

Author(s): K. Melcher.

Journal Name: Current Protein & Peptide Science

Volume 5 , Issue 4 , 2004

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Most proteins function as multiprotein complexes or interact with multiprotein complexes. Identification of protein-protein interactions in the context of their physiologically relevant complexes is therefore key to fully understand the cellular machinery. Here I discuss advances in chemical crosslinking methods that allow investigators to map direct subunit contacts in transient interactions with multimeric complexes. Methods discussed fall into two categories: (i) in vitro approaches with localized, inducible crosslinking reagents and (ii) in vivo approaches with unlocalized crosslinkers.

Keywords: multiprotein complexes, photoactivatable crosslinking, label transfer

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Article Details

Year: 2004
Page: [287 - 296]
Pages: 10
DOI: 10.2174/1389203043379701
Price: $65

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