Abstract
The bacterial RecA protein has been a model system for understanding how a protein can catalyze homologous genetic recombination. RecA-like proteins have now been characterized from many organisms, from bacteriophage to humans. Some of the RecA-like proteins, including human RAD51, appear to function as helical filaments formed on DNA. However, we currently have high resolution structures of inactive forms of the protein, and low resolution structures of the active complexes formed by RecA-like proteins on DNA in the presence of ATP or ATP analogs. Within a crystal of the E. coli RecA protein, a helical polymer exists, and it has been widely assumed that this polymer is quite similar to the active helical filament formed on DNA. Recent developments have suggested that this may not be the case.
Keywords: reca-dna filament?, reca protein, genetic recombination, reca-like proteins
Current Protein & Peptide Science
Title: What is the Structure of the RecA-DNA Filament?
Volume: 5 Issue: 2
Author(s): X. Yu, M. S. VanLoock, S. Yang, J. T. Reese and E. H. Egelman
Affiliation:
Keywords: reca-dna filament?, reca protein, genetic recombination, reca-like proteins
Abstract: The bacterial RecA protein has been a model system for understanding how a protein can catalyze homologous genetic recombination. RecA-like proteins have now been characterized from many organisms, from bacteriophage to humans. Some of the RecA-like proteins, including human RAD51, appear to function as helical filaments formed on DNA. However, we currently have high resolution structures of inactive forms of the protein, and low resolution structures of the active complexes formed by RecA-like proteins on DNA in the presence of ATP or ATP analogs. Within a crystal of the E. coli RecA protein, a helical polymer exists, and it has been widely assumed that this polymer is quite similar to the active helical filament formed on DNA. Recent developments have suggested that this may not be the case.
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Cite this article as:
Yu X., VanLoock S. M., Yang S., Reese T. J. and Egelman H. E., What is the Structure of the RecA-DNA Filament?, Current Protein & Peptide Science 2004; 5 (2) . https://dx.doi.org/10.2174/1389203043486883
DOI https://dx.doi.org/10.2174/1389203043486883 |
Print ISSN 1389-2037 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5550 |
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