Protein Extraction and Comparison of Stain Protocols for Analysis of Two-Dimensional Electrophoresis Gels

Author(s): Joana Silva, Ana Sofia Carvalho, Rui Vitorino, Pedro Domingues, Paula Teixeira, Paul Gibbs.

Journal Name: Protein & Peptide Letters

Volume 11 , Issue 2 , 2004

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Abstract:

Protein extraction and the proteome of Lactobacillus delbrueckii subsp. bulgaricus were studied using different stains. The reversible silver staining technique was shown to be more sensitive than the irreversible silver stain. Coomassie colloidal was demonstrated to be as sensitive as reversible silver stain; however, the Coomassie colloidal blue solution developed a higher background and for sample preparation was more time-consuming.

Keywords: Lactobacillus bulgaricus,, protein extraction,, 2-DE electrophoresis,, staining,

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Article Details

VOLUME: 11
ISSUE: 2
Year: 2004
Page: [189 - 194]
Pages: 6
DOI: 10.2174/0929866043478293
Price: $58

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