Seed Protein Variation Among Pepper (Capsicum Sp.) Genotypes Revealed By Maldi-Tof Analysis

Author(s): Marco Aurelio Caldas de Pinho Pessoa Filho, Carlos Bloch Junior, Danilo Fernandes da Silva Filho, Alexsandro Sobreira Galdino, Rodrigo Maranguape Silva da Cunha, Maria Aparecida Oliveira Alves, Thalles Barbosa Grangeiro.

Journal Name:Protein & Peptide Letters

Volume 11 , Issue 1 , 2004

Abstract:

A method for seed proteome analysis using MALDI-TOF mass spectrometry is described. The data were used to estimate the genetic diversity degree among twelve genotypes of pepper (Capsicum). The resulting spectra were converted into a binary matrix consisting of 23 protein data sets, and genetic similarity values were calculated with the FreeTree software and Jaccards coefficient of similarity. We have also been able to identify the presence of certain proteins in the extracts, by checking their masses on online databases.

Keywords: mass spectrometry, time of flight, germplasm, capsicum, genetic variability

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Article Details

VOLUME: 11
ISSUE: 1
Year: 2004
Page: [57 - 62]
Pages: 6
DOI: 10.2174/0929866043478482

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