Cytosolic Insulin-Binding Proteins Of Mouse Liver Cells

Author(s): Petr G. Lokhov, Sergei A. Moshkovskii, Olga M. Ipatova, Vladimir N. Prozorovskii.

Journal Name: Protein & Peptide Letters

Volume 11 , Issue 1 , 2004

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It has been recently shown that insulin retains its biological activity after receptor-directed internalization and it may affect the cell metabolism by interaction with cytosolic insulin-binding proteins (CIBPs). Using affinity chromatography combined with SDS-PAGE and MALDI-TOF mass-spectrometry we have identified 7 proteins from mouse liver cells that specifically bind to the insulin, including adenylate kinase 2 (25.6 kD), kinesin superfamily protein 20B (26.0 kD), hepatic arginase 1 (34.8 kD), fructosebisphosphate aldolase B (39.5 kD), 4-hydroxyphenylpyruvate dioxygenase (45.1 kD), betainehomocysteine methyl-transferase (45.0 kD) and KRIT1 (83.4 kD).

Keywords: insulin-binding protein, affinity chromatography, cytosol, liver cells

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Article Details

Year: 2004
Page: [29 - 33]
Pages: 5
DOI: 10.2174/0929866043478356

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