Identification of Sanguinarine as a Novel HIV Protease Inhibitor from High-Throughput Screening of 2,000 Drugs and Natural Products with a Cell-Based Assay

Author(s): Ting-Jen Cheng, David S. Goodsell, Chen-Chen Kan.

Journal Name: Letters in Drug Design & Discovery

Volume 2 , Issue 5 , 2005

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We identified a non-peptidomimetic HIV protease inhibitor sanguinarine through screening a small compound library with a high-throughput E. coli-based assay. In vitro, sanguinarine inhibits the HIV protease with an IC50 of around 13 μM. Based on docking results, binding modes of sanguinarine to the HIV protease are proposed.

Keywords: high-throughput screening, e. coli-based system, sanguinarine, hiv protease, hiv protease inhibitors, hiv protease precursor

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Article Details

Year: 2005
Page: [364 - 371]
Pages: 8
DOI: 10.2174/1570180054405811
Price: $58

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