A Cellular ELISA to Screen Lectin-Like Compounds for Cancer Cell Binding
C- P. Chang,
W- C. Cheng,
H- Y. Lei.
Lectins are carbohydrate-binding proteins that can modulate cellular activity through cell membrane glycoproteins. A cell-based binding was set up to screen the lectin-like substances in natural products. Lectins, after binding to tumor cells, are detected with a biotinylated carbohydrate-polyacrylamide complex by ELISA. Sugar specificity can also be determined within the assay.
Keywords: lectin, elisa, apoptosis, mitogenic
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