Synthesis and Evaluation of a Novel Gene Reporter Molecule: Detection of b-galactosidase Activity Using 19F NMR of a Fluorinated Vitamin B6 Conjugate+

Author(s): Jianxin Yu, Zhenyi Ma, Yingming Li, Kenneth S. Koeneman, Li Liu, Ralph P. Mason.

Journal Name: Medicinal Chemistry

Volume 1 , Issue 3 , 2005

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Abstract:

Gene therapy has emerged as a promising strategy for treatment of various diseases. However, widespread implementation is hampered by difficulties in assessing the success of transfection, in particular, the spatial extent of expression in the target tissue and the longevity of expression. Thus, the development of non-invasive reporter techniques based on appropriate molecules and imaging modalities may help to assay gene expression. We now report the design, synthesis and evaluation of a novel in vivo gene transfection reporter molecule 3-O-(β-D-galactopyranosyl)-6- fluoropyridoxol (GFPOL) using fluorinated vitamin B6 as the 19F NMR sensitive aglycone. GFPOL exhibits the following strengths as an in vivo 19F NMR gene expression reporter: (a) large chemical shift response to enzyme cleavage (Δd=8.00 ppm); (b) minimal toxicity for substrate or aglycone; (c) good water solubility; (d) good blood stability; (e) pH responsiveness of aglycone.

Keywords: galactosidase, f nmr, gene reporter, pyridoxol, ph

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Article Details

VOLUME: 1
ISSUE: 3
Year: 2005
Page: [255 - 262]
Pages: 8
DOI: 10.2174/1573406053765495
Price: $58

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