Structure-Function Relationships in Serine Protease-Bovine Pancreatic Trypsin Inhibitor Interaction
D. Krowarsch, M. Zakrzewska, A. O. Smalas and J. Otlewski
Affiliation: Institute of Biochemistry&Molecular Biology, University of Wroclaw, Tamka 2, 50-137 Wroclaw, Poland.
We report our progress in understanding the structure-function relationships for the interaction between BPTI and serine proteases. We focused on extensive mutagenesis of four crucial positions from the protease binding loop of BPTI. Selected variants were characterized by determination of association constants, stability parameters and structures of protease-inhibitor complexes.
Keywords: protein-protein recognition, serine protease, canonical protein inhibitor, protein stability
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