Preliminary X-Ray Crystallographic Analysis of Centrin from Ciliate Euplotes octocarinatus

Author(s): Xiao-Jing He, Liu Li, Hai Hou, Yaqin Zhao, Aihua Liang, Zihe Rao.

Journal Name: Protein & Peptide Letters

Volume 12 , Issue 6 , 2005

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Abstract:

Centrins are four-EF-hand Ca2+-binding proteins, which belong to the CaM super family. The centrin from ciliate Euplotes Octocarinatus has been expressed in Escherichia coli, purified and crystallized using the hanging-drop method. Rod-like crystals were grown and diffracted to 2.0 Å. The crystals belong to space group P222 and the unit-cell parameters are a=34.442 Å, b=48.954 Å, c=72.583 Å.

Keywords: centrin, crystallization, preliminary x-ray studies

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Article Details

VOLUME: 12
ISSUE: 6
Year: 2005
Page: [609 - 611]
Pages: 3
DOI: 10.2174/0929866054395842
Price: $65

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