Characterization of Expression and Stability of Recombinant Cystein-Rich Protein Human MT1A from Yeast

Title: Characterization of Expression and Stability of Recombinant Cystein-Rich Protein Human MT1A from Yeast

Volume: 12 Issue: 6

Author(s): Li Jie, Shao Kaifeng, Yao Dian, An Lin and Ru Binggen

Affiliation:

Keywords: human metallothionein a, secret expression, stability, polymerization, purification, pichia pastoris, yeast, function

Abstract: Metallothionein (MT) is the protein that has been shown to bind heavy metals, scavenge free radicals, protect DNA from radiation damage, and alleviate disease symptoms. However, only very limited success has been achieved in expression and production of active recombinant metallothionein. In this study, human metallothionein 1A (hMT1A) was transformed into yeast Pichia pastoris for expression with secretion of the protein into the medium. The expression system was optimized to obtain the targeted protein in active form at 335mg per litre culture. hMT1A showed the character of extreme instability in the experiment. High concentration, aeration and heavy metal ions are the main factors affecting hMT1A stability.

Cite this article as:

Jie Li, Kaifeng Shao, Dian Yao, Lin An and Binggen Ru, Characterization of Expression and Stability of Recombinant Cystein-Rich Protein Human MT1A from Yeast, Protein & Peptide Letters 2005; 12 (6) . https://dx.doi.org/10.2174/0929866054395734