Production of the Human-Beta-Defensin Using Saccharomyces cerevisiae as a Host

Author(s): Ingrid Cipakova, Eva Hostinova.

Journal Name: Protein & Peptide Letters

Volume 12 , Issue 6 , 2005

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Abstract:

Studies of the human defensins have been hampered by the lack of a simple expression system allowing for rapid production of functional peptide forms. Here, we describe a Saccharomyces cerevisiae AH22 expression system that meets that condition. The 42 amino acid form of human β-defensin-1 was expressed under the control of the ADH1 promoter. The optimum conditions for expression were determined and the stable maintenance of the pVT103L-hBD-1 chimeric vector in the yeast population was confirmed. Expressed hBD-1 was secreted into the medium (∼55 μg l-1) and purified using cation-exchange chromatography. Isolated defensin exhibited strong bactericidal effect on Escherichia coli ML-35p. We conclude that the expression system described here will be a useful tool where readily prepared and active forms of the human defensins are needed.

Keywords: antimicrobial peptide, human-beta-defensin-1 (hbd-1), s. cerevisiae ah, pvt vector, heterologous expression

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Article Details

VOLUME: 12
ISSUE: 6
Year: 2005
Page: [551 - 554]
Pages: 4
DOI: 10.2174/0929866054395761
Price: $65

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