Influence of Disulfide Bonds on the Conformational Changes and Activities of Refolded Phytase

Author(s): G. Y. Song, X. Y. Wang, M. Wang.

Journal Name: Protein & Peptide Letters

Volume 12 , Issue 6 , 2005

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Abstract:

Aspergillus sp. phytase contains five disulfide bonds. In order to elucidate their role, the reactivation and refolding of phytase in the absence and presence of dithiothreitol (DTT) was investigated. The results indicated that the disulfide bonds play an important role in the catalytic activity and conformational stability of the enzyme.

Keywords: phytase, refolding, disulfide bond, conformational stability, enzyme activity

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Article Details

VOLUME: 12
ISSUE: 6
Year: 2005
Page: [533 - 535]
Pages: 3
DOI: 10.2174/0929866054395752
Price: $65

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