Expression, Purification and Partial Characterization of the Krüppel- Associated Box (KRAB) from the Human ZNF2 Protein

Author(s): Vincenzo Rivieccio, Riccardo Mannini, Luigi Concilio, Sabato D'Auria, Carlo Pedone, Giovanna Grimaldi.

Journal Name: Protein & Peptide Letters

Volume 12 , Issue 6 , 2005

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Abstract:

The Kruppel-associated box (KRAB) domain is a potent transcription repression bipartite domain, shared by over 400 zinc finger proteins in humans, involved in the regulation of many functions. KRAB domains are both physically and functionally bipartite (A and B modules). The lone KRAB-A and composite KRAB-AB domains from the human ZNF2 protein were over-expressed as recombinant proteins in E. coli, isolated and purified to homogeneity to investigate their structure to function relationship.

Keywords: transcriptional repression, krab domain, zinc finger, fusion protein expression

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Article Details

VOLUME: 12
ISSUE: 6
Year: 2005
Page: [527 - 532]
Pages: 6
DOI: 10.2174/0929866054395833
Price: $65

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