Production of Minichaperone (sht GroEL191-345) and Its Function in the Refolding of Recombinant Human Interferon Gamma

Author(s): Yi-Xin Guan, Zheng-Zheng Fei, Man Luo, Shan-Jing Yao, Man-Gi Cho.

Journal Name: Protein & Peptide Letters

Volume 12 , Issue 1 , 2005

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Abstract:

The recombinant minichaperone sht GroEL191-345 was cultivated in a 3.7 L stirred bioreactor with the high yield of 216.2 mg / L broth. In the refolding of recombinant human interferon gamma (rhuIFN-γ) inclusion bodies, more than 2-3 fold enhancement in protein mass recovery and total activity were observed in the presence of free or immobilized minichaperone to the refolding buffer.

Keywords: minichaperone, sht groel191-345, cultivation, purification, refolding, recombinant human interferon gamma

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Article Details

VOLUME: 12
ISSUE: 1
Year: 2005
Page: [85 - 88]
Pages: 4
DOI: 10.2174/0929866053405995

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