Selection of a Catalytically Active Enzyme In Vivo Via Phage Display of its Product

Author(s): T. Taki, S. Fujita, K. Taira.

Journal Name: Letters in Drug Design & Discovery

Volume 2 , Issue 6 , 2005

Abstract:

We have developed a general strategy for the selection of genes that encode active enzymes. In our strategy, the product of a catalytic reaction in vivo is displayed on secreted phage via the g8p protein. The subsequent affinity-capture of the product in vitro results in enrichment of the gene that encodes an active enzyme. In a model study, we succeeded in the selection of a gene for biotin protein ligase.

Keywords: biotin protein ligase, in vivo selection, phage display, enzyme catalysis

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Article Details

VOLUME: 2
ISSUE: 6
Year: 2005
Page: [475 - 478]
Pages: 4
DOI: 10.2174/1570180054771482
Price: $58