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Letters in Drug Design & Discovery

Editor-in-Chief

ISSN (Print): 1570-1808
ISSN (Online): 1875-628X

Selection of a Catalytically Active Enzyme In Vivo Via Phage Display of its Product

Author(s): T. Taki, S. Fujita and K. Taira

Volume 2, Issue 6, 2005

Page: [475 - 478] Pages: 4

DOI: 10.2174/1570180054771482

Price: $65

Abstract

We have developed a general strategy for the selection of genes that encode active enzymes. In our strategy, the product of a catalytic reaction in vivo is displayed on secreted phage via the g8p protein. The subsequent affinity-capture of the product in vitro results in enrichment of the gene that encodes an active enzyme. In a model study, we succeeded in the selection of a gene for biotin protein ligase.

Keywords: biotin protein ligase, in vivo selection, phage display, enzyme catalysis


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