Cloning, Expression, Purification, Characterization, Crystallization and X-Ray Diffraction of Bifunctional Pyrimidine Deaminase/Reductase from Shigella flexneri 2a

Title: Cloning, Expression, Purification, Characterization, Crystallization and X-Ray Diffraction of Bifunctional Pyrimidine Deaminase/Reductase from Shigella flexneri 2a

Volume: 14 Issue: 9

Author(s): Daopeng Yuan, Qihai Wang, Wei Gao, Fanyi Sheng, Zhanyu Zhang, Qingyu Lu, Huaixing Cang and Ruchang Bi

Affiliation:

Keywords: RibD, Shigella flexneri 2a, bifunctional pyrimidine deaminase/reductase, riboflavin biosythesis, expression, crystallization

Abstract: Bifunctional pyrimidine deaminase/reductase (RibD) plays an important role during riboflavin biosynthesis in many microorganisms. The 40.4 kDa RibD from Shigella flexneri 2a has been cloned, expressed, purified and characterized. Three Crystals of RibD have been obtained by the hanging-drop technique at 291 K using PEG 20k or NaCl as precipitant. The RibD crystal using PEG 20k as precipitant diffracted to 2.5Å.

Cite this article as:

Yuan Daopeng, Wang Qihai, Gao Wei, Sheng Fanyi, Zhang Zhanyu, Lu Qingyu, Cang Huaixing and Bi Ruchang, Cloning, Expression, Purification, Characterization, Crystallization and X-Ray Diffraction of Bifunctional Pyrimidine Deaminase/Reductase from Shigella flexneri 2a, Protein & Peptide Letters 2007; 14 (9) . https://dx.doi.org/10.2174/092986607782110347