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Protein & Peptide Letters

Editor-in-Chief

ISSN (Print): 0929-8665
ISSN (Online): 1875-5305

A Surface Immobilization Method of Endoglucanase from Cellulomonas biazotea Mutant Improved Catalytic Properties of Biocatalyst During Processing

Author(s): Muhammad Ibrahim Rajoka, Yasmin Zia and Khalil-ur-Rehman

Volume 14, Issue 7, 2007

Page: [734 - 741] Pages: 8

DOI: 10.2174/092986607781483886

Price: $65

Abstract

Purified endoglucanase from C. biazotea mutant 51SMr was successfully immobilized on Eudragit L-100, with 75 % yield of immobilization. This method improved the kinetic and thermodynamic properties of the enzyme. Immobilization significantly decreased entropy and enthalpy of inactivation of biocatalyst and made it functionally and thermodynamically more stable and reusable compared to free one.

Keywords: Eudragit L-100, immobilization kinetics, melting point, substrate binding energy, thermodynamics, transition state stabilization

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