A Surface Immobilization Method of Endoglucanase from Cellulomonas biazotea Mutant Improved Catalytic Properties of Biocatalyst During Processing

Author(s): Muhammad Ibrahim Rajoka, Yasmin Zia, Khalil-ur-Rehman.

Journal Name: Protein & Peptide Letters

Volume 14 , Issue 7 , 2007

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Abstract:

Purified endoglucanase from C. biazotea mutant 51SMr was successfully immobilized on Eudragit L-100, with 75 % yield of immobilization. This method improved the kinetic and thermodynamic properties of the enzyme. Immobilization significantly decreased entropy and enthalpy of inactivation of biocatalyst and made it functionally and thermodynamically more stable and reusable compared to free one.

Keywords: Eudragit L-100, immobilization kinetics, melting point, substrate binding energy, thermodynamics, transition state stabilization

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Article Details

VOLUME: 14
ISSUE: 7
Year: 2007
Page: [734 - 741]
Pages: 8
DOI: 10.2174/092986607781483886
Price: $58

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