Ultrasensitive Staining-Free Protein Detection After PAA Gel Electrophoresis Using Deep UV Fluorescence

Author(s): Eugene Riaplov, Qiang Li, Stefan Seeger.

Journal Name: Protein & Peptide Letters

Volume 14 , Issue 7 , 2007

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Abstract:

We present the observation of separated protein bands after polyacrylamide (PAA) gel electrophoresis based on the staining-free detection of their ultra violet (UV)-induced fluorescence employing deep UV confocal fluorescence microscopy. Mixtures of the three biological compounds β-Galactosidase (from Escherichia coli), apo-Transferrin (bovine) and bovine serum albumin (BSA) have been separated and a staining free detection limit below 80 pg (7.0x108 molecules) per band has been achieved. This corresponds to ∼270 molecules in the detection volume for confocal microscopy.

Keywords: Native fluorescence, deep-UV, polyacrylamide, gel electrophoresis, staining free

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Article Details

VOLUME: 14
ISSUE: 7
Year: 2007
Page: [712 - 715]
Pages: 4
DOI: 10.2174/092986607781483787
Price: $58

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